Forensiker erwähnen das (angebliche) Phänomen der menschlichen Selbstentzündung nur sehr selten.
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Investigation into a turning point of German juridical proceedings. Here, we focus on the multiple psychiatric decisions on the mental state of a juvenile, pedophile, homosexual serial killer, and the influence of those decisions on the trial.
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Here is that rare thing, a good popular book on forensic entomology that is also an illuminating read on forensic science itself and on the art of being an expert witness.
Read MoreThe quantitative alteration of the DNA content in strangulation marks is an artefact
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Read MoreVariability of the mitochondrial loci nt00073 and nt16519 in populations of Germany, Syria, Cameroon, Japan, Vietnam and Peru—a study using the RFLP and Light Cycler™ technique
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Read MoreY-chromosomal short tandem repeat haplotypes at the loci DYS393, DYS19, DYS392, and DYS385-I/II, DYS390, DYS389-I/II, and DYS391 in a Filipino population sample
Source: J Forensic Sci (2001) 46(5): 1250–3.
Miranda JJ, Benecke M, Hidding M, Schmitt C
The article as .pdf
Population: Male population sample (n = 106) from the Metro Manila area (largest urban center in the Philippines).
Keywords: forensic science, forensic DNA typing, short tandem repeat (STR), Y chromosome, genetic fingerprint, Philippines
Whole blood samples were obtained from 106 unrelated male individuals living in Metro Manila, Philippines, through the Department of Health, Manila. DNA was extracted by isopropanol fractionation-sodium iodide precipitation (4) and quantified by spectrophotometry. Nine Y-chromosomal short tandem repeats (STR’s) were analyzed from a population sample of 106 unrelated males by means of a quadruplex PCR (DYS393, DYS19, DYS392, DYS385-I/II) and a triplex PCR (DYS390, DYS389-I/II, and DYS391).
Primers were Cy5-labeled, and based on sequences described by Kayser et al. (1). PCR products were separated on ReproGel™ High Resolution polyacrylamide gels, and laser-detected by an ALFexpress sequencer (Amersham Pharmacia Biotech). Allelic ladders, and nomenclature were standardized against allelic ladders from P. de Knijff (Leiden), L. Roewer (Berlin), J. Edelmann (Leipzig), and P. Schneider (Mainz).
Discrimination capacity for the nine-loci system was 83%. Gene diversity was calculated following Kayser et al. (3). Frequencies of the individual alleles are shown in Table 2. Haplotype data (88 distinct haplotypes, 75 of which were unique) are given in Table 1. Gene diversity values ranged between 0.37 for DYS91 and 0.94 for DYS385, which is similar to frequences reported elsewhere (2,3).
Acknowledgments
The authors wish to acknowledge the assistance of Rubigilda Paraguison and Edith Tria in the collection and preparation of the samples. Dr. Miranda was supported by a fellowship of the Deutscher Akademischer Austauschdienst (DAAD, German Academic Exchange Service), Federal Republic of Germany.
References
1. Kayser M, Caglia A, Corach D, Fretwell N, Gehrig C, Graziosi G, et al. Evaluation of the Y-chromosomal STRs: a multicenter study. Int J Legal Med 1997;110:125–33.
2. Pestoni C, Cal ML, Lareu MV, Rodriguez-Calvo, Carracedo A. Y chromosome STR haplotypes: genetic and sequencing data of the Galician population (NW Spain). Int J Legal Med 1998;112:15–21.
3. Rossi E, Rolf B, Schürenkamp M, Brinkmann B. Y-chromosome STR haplotypes in an Italian population sample. Int J Legal Med 1998;112: 78–81.
4. Wang L, Hirayasu K, Ishizawa M, Kobayashi Y. Purification of genomic DNA from human whole blood by isopropanol fractionation with concentrated NaI and SDS. Nucleic Acids Res 1994;22:1774 –5.
Homicide in the Ukraine
Skull & bone fragments
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by ants 🐜
Insektenbefall am Leichnam
Wissensquelle der Gerichtsmedizin
Alteration of DNA content in strangulation marks
Is an artefact
Forensic DNA Lab
University of the Philippines, Diliman, Manila; Ho Chi Minh City
Warum Insekten essen keine gute Idee ist
Radio-Interview, 2023
A homicide in the Ukraine
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For several years Y-chromosomal microsatellites (short tandem repeats, STRs) have been well established in forensic practice. In this context, the genetic characteristics of the Y chromosome (i.e. its paternal inheritance and lack of recombination) render STRs particularly powerful.
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To permit quick identification of arthropods, random amplified polymorphic DNA typing (RAPD) was used to support classical morphological and medico-legal analysis of maggots on a human corpse.
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Wounds of living persons are a potential target for the same flies that live, or feed early on corpses. This can lead to complications in estimation of PMI but also allows to determine additional information that might be valuable in a trial, or during the investigations.
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