Comments on the historical background & Interview
Read MoreInsects Under The Skin? Ziploc And Matchbox Evidence In The Expert Forensic Stain Laboratory
The idea of insects living inside and on the (human) body literally drives people affected by the thought crazy. We receive samples containing alleged insect stain evidence on a regular basis which on examination mostly turn out to be non-animal fragments of skin, hair, fibers et cetera.
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Book review on A Fly for the Prosecution by Lee Goff
Read MoreMind Your Decompositional Assumptions
Decompositional processes depend on temperature, humidity, and light, as well as on animals that can access the body. In low-level cases, suicides, or cases of self-neglect especially, these factors are not always taken into consideration because a full investigation may not have taken place.
Read MoreThe hunt for Hitler's teeth
How one forensic biologist stumbled across the only remaining body parts of Adolf Hitler… in a floppy-disc case in Moscow.
Read MoreDNA typing in forensic medicine and in criminal investigations: a current survey
A brief overview is given of past and present DNA typing and the establishment of forensic DNA databases in Europe.
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We have not been able to distinguish different isolates from the nematode Caenorhabditis elegans by morphological means. However, they differ on the molecular level and strains from several geographic regions can be identified with the help of "genetic fingerprints" using the oligonucleotide probe (GTG)5.
Read MoreAllele frequency distributions of the polymorphic STR loci HUMVWA, HUMFES, HUMF13A01 and the VNTR D1S80 in a Filipino population from Metro Manila
Allele frequency distributions at the short tandem repeat (STR) loci HUMVWA, HUMFES, HUMF13A01 and of the variable number of tandem repeat (VNTR) locus D1S80 were determined in a Filipino population from Metro Manila (103 individuals) by use of the polymerase chain reaction (PCR) followed by polyacrylamide gel electrophoresis (PAGE).
Read MoreShort tandem repeat (STR) locus HUMD8S306 in a large population sample from Germany
Applied DNA typing in medico-legal investigations, in criminalistic practice, and in paternity cases often relies on high inclusion and exclusion probabilities.
Read MoreInhibitory learning and memory in the topshell (Monodonta lineata)
One hundred eighty topshells (Monodonta lineata) were exposed to inhibitory learning procedures.
Read MoreDetection and quantification of Proporxur in the succession of insects of medico-legal importance
To detect and quantify the amount of Propoxur in insects of forensic importance and search for entomotoxicologic indicators, the HPLC (High Performance Liquid Chromatography) technique was used on individuals collected during the ecological succession associated with the corpses of rabbits.
Read MoreTwo homosexual pedophile sadistic serial killers
From a criminalistic and legal perspective, we report 2 cases of homosexual pedophile serial killers who share certain characteristics.
Read MoreHomosexual Pedophile Serial Killer Juergen Bartsch (1946-1976)
Report on a fully confessing, very young serial killer who exactly detailed his crimes und intentions to the police, und several psychiatrists.
Read MoreMurder most putrid
Here is that rare thing, a good popular book on forensic entomology that is also an illuminating read on forensic science itself and on the art of being an expert witness.
Read MoreThe quantitative alteration of the DNA content in strangulation marks is an artefact
It is frequently difficult to prove the vitality in cases of fatal hanging. Statistical analysis demonstrated a significant correlation between elevated DNA content and reduced water content of strangulation marks.
Read MoreY-chromosomal short tandem repeat haplotypes at the loci DYS393, DYS19, DYS392, and DYS385-I/II, DYS390, DYS389-I/II, and DYS391 in a Filipino population sample
Source: J Forensic Sci (2001) 46(5): 1250–3.
Miranda JJ, Benecke M, Hidding M, Schmitt C
The article as .pdf
Population: Male population sample (n = 106) from the Metro Manila area (largest urban center in the Philippines).
Keywords: forensic science, forensic DNA typing, short tandem repeat (STR), Y chromosome, genetic fingerprint, Philippines
Whole blood samples were obtained from 106 unrelated male individuals living in Metro Manila, Philippines, through the Department of Health, Manila. DNA was extracted by isopropanol fractionation-sodium iodide precipitation (4) and quantified by spectrophotometry. Nine Y-chromosomal short tandem repeats (STR’s) were analyzed from a population sample of 106 unrelated males by means of a quadruplex PCR (DYS393, DYS19, DYS392, DYS385-I/II) and a triplex PCR (DYS390, DYS389-I/II, and DYS391).
Primers were Cy5-labeled, and based on sequences described by Kayser et al. (1). PCR products were separated on ReproGel™ High Resolution polyacrylamide gels, and laser-detected by an ALFexpress sequencer (Amersham Pharmacia Biotech). Allelic ladders, and nomenclature were standardized against allelic ladders from P. de Knijff (Leiden), L. Roewer (Berlin), J. Edelmann (Leipzig), and P. Schneider (Mainz).
Discrimination capacity for the nine-loci system was 83%. Gene diversity was calculated following Kayser et al. (3). Frequencies of the individual alleles are shown in Table 2. Haplotype data (88 distinct haplotypes, 75 of which were unique) are given in Table 1. Gene diversity values ranged between 0.37 for DYS91 and 0.94 for DYS385, which is similar to frequences reported elsewhere (2,3).
Acknowledgments
The authors wish to acknowledge the assistance of Rubigilda Paraguison and Edith Tria in the collection and preparation of the samples. Dr. Miranda was supported by a fellowship of the Deutscher Akademischer Austauschdienst (DAAD, German Academic Exchange Service), Federal Republic of Germany.
References
1. Kayser M, Caglia A, Corach D, Fretwell N, Gehrig C, Graziosi G, et al. Evaluation of the Y-chromosomal STRs: a multicenter study. Int J Legal Med 1997;110:125–33.
2. Pestoni C, Cal ML, Lareu MV, Rodriguez-Calvo, Carracedo A. Y chromosome STR haplotypes: genetic and sequencing data of the Galician population (NW Spain). Int J Legal Med 1998;112:15–21.
3. Rossi E, Rolf B, Schürenkamp M, Brinkmann B. Y-chromosome STR haplotypes in an Italian population sample. Int J Legal Med 1998;112: 78–81.
4. Wang L, Hirayasu K, Ishizawa M, Kobayashi Y. Purification of genomic DNA from human whole blood by isopropanol fractionation with concentrated NaI and SDS. Nucleic Acids Res 1994;22:1774 –5.
Homicide in the Ukraine
Skull & bone fragments
Post mortem damages
by ants 🐜
Insektenbefall am Leichnam
Wissensquelle der Gerichtsmedizin
Alteration of DNA content in strangulation marks
Is an artefact
Forensic DNA Lab
University of the Philippines, Diliman, Manila; Ho Chi Minh City
Warum Insekten essen keine gute Idee ist
Radio-Interview, 2023
A homicide in the Ukraine
In an apartment, bone fragments were found in a fireplace. Furthermore, a varnished skull was found elsewhere in the same apartment.
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Having solved the last technical hurdles to extract DNA information from virtually any biological material, forensic biologists now have to ponder the ethical and social questions of using information from exonic DNA.
Read MoreAsian online Y-STR Haplotype Reference Database
For several years Y-chromosomal microsatellites (short tandem repeats, STRs) have been well established in forensic practice. In this context, the genetic characteristics of the Y chromosome (i.e. its paternal inheritance and lack of recombination) render STRs particularly powerful.
Read MoreSix forensic entomology cases: description and commentary
Several cases are reported which show that a wide range of applications in medico-legal questions and hygiene together or apart from estimating the PMI can be answered by use of Forensic Entomology techniques including close observation of larval development.
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